1.0 M HEPES Buffer pH 7.2
Quick Overview for 1.0 M HEPES Buffer pH 7.2 (ABIN925581)
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Supplier Product No.
- mb-062-0100
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Supplier
- Rockland
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Purpose
- 1.0 M HEPES Buffer pH 7.2
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Purification
- 1.0 HEPES pH 7.2 was aseptically filtered through a Millipore 0.22 micron filter into clean, pre-sterilized containers. 1.0 M HEPES pH 7.2 was tested on trypticase soy agar for 24 hours, 48 hours and 72 hours and was found to be negative for bacteria.
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Sterility
- Sterile filtered
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Blocking Buffer for Fluorescent Western Blotting
CA, ELISA, IF, IHC, IP, MA, WB 1 X
10X TTBS Buffer pH 7.5ELISA, WB 10 X
Fluoromount-G®FISH, ICC, IHC (fro), IHC (p), IHC (wm), PLA, TUNEL
10X PBS Buffer pH 7.2ELISA, FACS, IF, WB 10 X
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Application Notes
- 1.0 M HEPES pH 7.2 is a concentrated stock solution and should be diluted appropriately with molecular biology grade water or equivalent to its final working concentration. This buffer consists of 1.0 M HEPES at a pH of 7.2 and was prepared using ultra p
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1.0 M
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Handling Advice
- Some salts may precipitate out of solution at lower temperature. Allow buffer to equilibrate to room temperature
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Storage
- RT,4 °C
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Storage Comment
- Store container at room temperature (18° to 26° C) prior to opening. If desired, the solution may be stored at 4° C or less. Some salts may precipitate out of solution at lower temperature. Allow buffer to equilibrate to room temperature (18° to 26° C) Expiration date is six (6) months from date of receipt.
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Expiry Date
- 6 months
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Background
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Synonyms: 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid
Background: HEPES is a useful buffer for pH 's ranging from 6.8-8.2. This makes HEPES ideal for cell culturing because of its usefulness in maintaining pH changes in presence of carbon dioxide production from cellular respiration. 1.0 M HEPES pH 7.2 is ideal for inve
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